3 edition of Adaptive Hexokinase isozymes of rat epididymal adipose tissue found in the catalog.
Adaptive Hexokinase isozymes of rat epididymal adipose tissue
Robert J. Hansen
Written in English
|Statement||Robert John Hansen.|
|LC Classifications||Microfilm 23812|
|The Physical Object|
|Pagination||vi, 63 l.|
|Number of Pages||63|
|LC Control Number||94895003|
Influence of Dietary Fat Level on the Enzymatic and Lipogenic Adaptations in Adipose Tissue of Meal-fed Rats Article (PDF Available) in Journal of Nutrition 91(2) March with 25 Reads. A new and highly sensitive fluorimetric assay for hexokinase (HK) isoenzymes which requires only 5 μg. of tissue protein is described. This assay has been used to examine the alterations in the HK isoenzymes of rat skeletal muscle and adipose tissue under a variety of physiologic conditions. In both tissues, HK-II declined progressively with increasing age, reaching plateau levels in animals Cited by:
Isozymes of mammalian hexokinase. Four distinct isozymes of hexokinase were separated by ion exchange chromatography (González et al., ) or electrophoresis (Katzen and Schimke, ) of extracts from various mammalian lly these are referred to as the Type I, Type II, Type III and Type IV isozymes, with the latter commonly called `glucokinase'.Cited by: Leveille GA. The reversal of glycolysis in rat epididymal adipose tissue. Life Sci. Apr 15; 6 (8)– Leveille GA, Hanson RW. Adaptive changes in enzyme activity and metabolic pathways in adipose tissue from meal-fed rats. J Lipid Res. Jan; 7 (1)– LOWRY OH, PASSONNEAU by:
The effects of dexamethasone and growth hormone on hexokinase (HK) isoenzymes and glucose utilization were studied in adipose tissue from young rats. Dexamethasone ( mg./kg. twice a day) caused hyperinsulinemia with normal blood glucose and marked diminution of adipose tissue HK-II after twenty-four and forty-eight hours. HK-I activity was reduced after twenty-four hours Cited by: Detection of Type III Hexokinase in Rat Tissue Extracts by Immunoblotting Freshly harvested rat tissues were minced on ice and homogenized in a buffer (1 ml buffer/ mg of tissue) consisting of 50 m m sodium phosphate, pH , 10 m m Glc, 10 m m thioglycerol, and % (v/v) Triton X, using a tightly fitting glass tissue homogenizer.
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TABLE I APPARENT K, VALUES AND SEPARATE ACTIVITIES OF THE TWO DISTINCT HEXOKINASES IN EPIDIDYMAL ADIPOSE TISSUE FROM FASTED AND CARBOHYDRATE-FED RATS The values presented are estimated from the data in Fig. 2 (see the text).Cited by: 1. Endocrinology. Dec;81(6) Properties of adaptive hexokinase isozymes of the rat.
Hansen R, Pilkis SJ, Krahl ME. PMID: Cited by: The isozymes of hexokinase in surgical specimens of human subcutaneous adipose tissue were separated by elution from DEAE-cellulose with linear KCl gradients at pH Two peaks of activity were found: Peak 1 eluted at M KCl, and Peak 2 at M : Victor R.
Lavis. Author: Bernstein RS. Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N.Y.) [01 Nov(2)]Cited by: 2. The effect of anti-insulin serum and alloxan diabetes on the distribution and multiple forms of hexokinase in lactating rat mammary gland Biochem J () Borrebaek, B.
Spydevold, O. The effects of insulin and glucose on mitochondrial-bound hexokinase activity of rat epididymal adipose tissue Diabetologia 5: () Cited by: In mammals there are four hexokinase isozymes with different tissue distributions.
Because the isozymes have different kinetic features, this has permitted them to become optimally adapted to the needs of the specific tissue in which they are the major isozyme. Isozymes of mammalian hexokinase Four distinct isozymes of hexokinase were separated by ion exchange chromatography (González et al., ) or electrophoresis (Katzen and Schimke, ) of extracts from various mammalian tissues.
Generally these are referred to as the Type I, Type II, Type III and Type IV isozymes, with theCited by: The Allosteric Regulation of Hexokinase C from Amphibian Liver * (Received for publication, March 1, ) TITO URETA Hexokinase A and hexokinase B are those isozymes eluting in the first and second position of the DEAE cellulose the same inhibition feature of rat hexokinase C has been found.
Each of the low Km hexokinase types designated I, II, and III has been purified approximately to fold from one or more rat tissues (Type I from brain and kidney, Type II from skeletal muscle and epididymal fat pad, and Type III from liver). Each purified enzyme type, regardless of tissue source, has.
Adaptive Hexokinase isozymes of rat epididymal adipose tissue [microform]: effects of insulin and utilizable energy source / Article Hexose-ATP phosphotransferases: Comparative aspects. The effect of fasting on the lactate dehydrogenase isozyme distribution pattern of rat epididymal adipose tissue as demonstrated by starch-gel electrophoresis.
Horizontal electrophoresis was run by using a starch concentration of lO% in 25 mM Tris-HC1 (pH 8), maintained at 4for by: 3. A hexokinase, with a low Michaelis constant, not previously described, has been found in extracts of human and dog liver but not of rat liver.
Earlier reports are contradicted in that glucokinase occurs in extracts of liver from well-nourished humans and dogs; it Cited by: Influence of Periodicity of Eating on the Activity of Various Enzymes in Adipose Tissue, Liver and Muscle of the Rat ' Article (PDF Available) in Journal of Nutrition 96(1) September with.
Kowalski TJ, Wu GY, Watford M () Rat adipose tissue amino acid metabolism in vivo as assessed by microdialysis and arteriovenous techniques. Am J Physiol E–E PubMed Google Scholar Kozak LP () Brown fat and the myth of diet-induced by: Cyclic AMP metabolism in adipose tissue of exercise-trained rats Article (PDF Available) in Journal of Lipid Research 19(6) September with 56 Reads How we measure 'reads'.
Keith Tornheim, in Encyclopedia of Endocrine Diseases (Second Edition), Hexokinase and glucose transport. Hexokinase is nominally the first step in glycolysis, but it is not the major point of regulation because glucose 6-phosphate is a branch point leading to glycogen and to the pentose phosphate pathway.
Importantly, glucose 6-phosphate is an inhibitor of hexokinase, so if the other. Control of Lipogenesis in Adipose Tissue of Fasted and Fed Meal-eating Rats Article (PDF Available) in Journal of Nutrition 92(4) September with 26 Reads How we measure 'reads'.
Insulin is a major anabolic hormone for most mammalian species. The hormonal potency of insulin results, to a large extent, from its ability to regulate target cells at a variety of cellular : I.
Goldfine, F. Purrello, R. Vigneri. isozymes (22), it was desirable to examine only the highly puri- fied native isozymes. Figs. 1 and 2 show the results with two yeast hexokinase isozymes, Pr and PII (23), at pH In Part A of these experiments, magnesium was maintained at 10 mM in excess of the ADP and ATP.
At this concentration. INTRODUCTION. Brain hexokinase, hexokinase I (ATP: D-hexose 6-phosphotransferase, EC ), is thought to be the pacemaker of glycolysis in brain tissue and the erythrocyte ().The cDNAs for hexokinases from a variety of tissues, including yeast (3, 4), human kidney (), rat brain (), rat skeletal muscle (), rat liver (8, 9), and mouse hepatoma have been cloned, and.
The tissue distribution of glucokinase (ATP:D-hexose 6-phosphotransferase, EC ) was examined by protein blotting analysis. Antibodies raised against rat liver glucokinase recognized a single protein subunit with an apparent Mr of 56, on nitrocellulose blots of cytosol protein from liver, separated by sodium dodecyl sulfate/polyacrylamide gel electrophoresis.Alexander, N.
M., R. Scheig, and G. Klatskin: Effects of prolonged ingestion of glucose or ethanol on fatty acid synthesis by mitochondria and cell sap of rat liver and adipose tissue.J. Lipid Res. 7, (). PubMed Google ScholarCited by: